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24500 a Bergey's manual of determinative bacteriology / c [edited by] John G. Holt .. [and others].
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500 a Includes index.
6500 a Bacteria v Classification. 0 http://id.loc.gov/authorities/subjects/sh85010865.
6500 a Bacteriology 0 http://id.loc.gov/authorities/subjects/sh85010864 v Terminology. 0 http://id.loc.gov/authorities/subjects/sh99001799.
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7001 a Holt, John G. 0 http://id.loc.gov/authorities/names/n80144855.
7300 a Shorter Bergey's manual of determinative bacteriology.
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Identification flow charts Bergey’s Manual of Determinative Bacteriology All of the unknowns will fall into the following groups in Bergey's Manual of Determinative Bacteriology (The pink book on the shelf in the laboratory). GROUP 4 Description: Gram Negative, Aerobic/Microaerophilic rods and cocci Key differences are: pigments/fluorescent, motility, growth requirements, denitrification, morphology, and oxidase, read Genera descriptions Examples: Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter GROUP 5 Description: Facultatively Anaerobic Gram negative rods Key differences are: growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions Examples : Family Enterobacteriaceae and Vibrionaceae GROUP 17 Description: Gram-Positive Cocci Key differences are: oxygen requirements, morph., growth requirements (45°C and supplements), read Genera descriptions Examples: Micrococcus, Staphylococcus, Streptococcus, Enterococcus, Lactococcus, Aerococcus GROUP 18 Description: Endospore-Forming Gram positive rods and cocci Key differences are: oxygen requirements, motility, morph, catalase Examples: Bacillus, Clostridium GROUP 19 Description: Regular, Nonsporlating Gram positive rods Key differences are: morph., oxygen require, catalase Examples: Lactobacillus, Listeria GROUP 20 Description: Irregular, Nonsporlating Gram-positive rods Key differences are: catalase, motility, morph., read Genera descriptions Examples: Actinomyces, Corynebacterium, Arthrobacter, Propionibacterium GROUP 21 Description: Weakly Gram-Positive Nonsporlating Acid Fast Slender Rods Key differences are: acid fast, growth Examples: Mycobacterium 1 Identification flow charts Differentiation via Gram stains and cell morphology. Gram Stain & Morphological Flowchart Some Examples Gram Positive Cocci Bacilli Filamentous Round in clusters & tetrads: Staphylococcus Micrococcus Peptococcus Oval shape in Chains: Streptococcus Peptostreptococcus Enterococcus Club-shaped and/or in palisades: Corynebacterium Listeria Erysipelothrix Mycobacterium (Acid Fast) Propionobacterium Spore-bearing, large, uniform: Bacillus Clostridium Extensive branching: Actinomyces Arachnia Nocardia (partially acid-fast) Branching rudimentry or absent: Erysipelothrix Lactobacillus Eubacterium Streptomyces Gram Negative Cocci Neisseria Veillonella Bacilli Medium-sized cocco-bacillary: Acinetobacter Moraxella Tiny-coccobacillary: Brucella Bordetella Bacteroides Pleomorphic coccobacillary & filamentous: Haemophilus Bacteroides Pasteurella Francisella Actinobacillus Eikenella Cardiobacterium Flavobacterium 2 Exaggerated pointed ends: Fusobacterium nucleatum Coiled & sphero-plastic: Streptobacillus Fusobacterium Curved, comma-shaped: Vibrio Campylobacter Spirillium Uniformly Bacillary: Enterobacteriaceae Pseudomonas Aeromonas Alcaligenes Chromobacterium Identification flow charts Gram Positive Rods ID Flowchart Gram Positive Rods Bacillus spp. Clostrididium spp. Corynebacterium spp. Lactobacillus spp. Mycobacterium spp. Mycobacterium smegmatis Spore Forming + + - Corynebacterium spp. Lactobacillus spp. Mycobacterium spp. Bacillus spp. Clostridium spp Acid Fast Strict Anaerobes Corynebacterium spp Lactobacillus spp. - + Clostridium spp. Bacillus spp. You sure you have this? Go to Bergey's. See separate flowchart. Catalase + - Corynebacterium spp. Lactobacillus spp. Starch Hydrolysis Glucose Ferm. Activity + Corynebacterium kutsceri Acid Acid & Gas Corynebacterium xerosis Lactobacillus fermenti Lactobacillus casei Lactobacillus delbrueckii Mannitol + Lactobacillus casei 3 - Lactobacillus delbrueckii Identification flow charts Bacillus spp. ID Flowchart Bacillus B. subtilis B. cereus B. megaterium B. stearothermophilus B. polymyxa B. mycoides B. macerans B. thuringiensis B. macquariensis B. licheniformis B. lentus B. alvei B. anthracis B. alcalophilus B. coagulans B. brevis B. circulans B. firmus B. pantothenticus Starch Hydrolysis (amylase) (makes insecticidal protein) B. mycoides B. azotoformans B. larvae B. pasteurii (grows at 55°C, rest no) VP + B. subtilis B. cereus B. polymyxa B. mycoides B. thuringiensis B. licheniformis B. alvei B. anthracis B. coagulans B. megaterium B. stearothermophilus B. macerans B. pantothenticus B. macquariensis B. lentus B. alcalophilus B. badius B. brevis B. circulans B. subtilis B. polymyxa B. lichenifomis B. alvei B. coagulans (colony has a rhizoidal appearance) B. popilliae Catalase (spore is not round, rest are) B. azotoformans Swollen Cell Swollen Cell (containing spore) (containing spore) - B. stearothermophilus B. megaterium (growth at 55°C-rest neg.) + (citrate pos.) B. macerans B. pantothenticus B. macquariensis B. lentus B. badius (citrate neg.) (acid from glucose) B. marinus (acid from glucose) Nitrate Reduction 6.5% NaCl Growth B. coagulans B. licheniformis B. subtilis B. insolitus (oval spore, rest are round) + - B. pasteurii B. sp sphaericus Citrate + - B. sphaericus B. pasteurii B. laterosporus B. alcalophilus B. brevis B. circulans (Motile - It is this one ) B. larvae (gelatinase pos.) B. popilliae (gelatinase neg.) B. insolitus B. laterosporus B. pasteurii B. marinus B. sphaericus (catalase neg.-rest pos.) B. cereus Citrate + + B. badius + - (round spore, rest are oval) B. popilliae B. pasteurii B. lentimorbus B. azotoformans B. badius B. insolitus B. larvae B. laterosporus (add creatine as catalyst w/ reagents) + B. thuringiensis (VP is pos., rest are neg.) B. marinus B. sphaericus B. schlegelii + (the width) (non-motile) B. pumilus - Cell Diamenter ! 1'm B. anthracis spp. B. polymyxa (manitol pos.) B. alvei (manitol neg.) + - B. macerans B. macquariensis B. alcalophilus B. brevis B. circulans B. pantothenticus (acid via arabinose neg.) B. circulans (acid via arabinose pos.) 6.5% NaCl Growth + B. subtilis (no growth at 55°C) Acid from Arabinose B. coagulans - B. licheniformis B. macquariensis (growth at 55°C) (methyl red pos.) B. brevis (methyl red neg.) 4 B. macerans + (gas from glucose pos.) B. alcalophilus (gas from glucose neg., no growth < pH 7.0) B. circulans (gas from glucose neg., growth < pH 7.0) Identification flow charts Gram Positive Cocci ID Flowchart Gram Positive Cocci Streptococcus spp. (see Bergey’s) Micrococcus s pp. Staphylococcus spp. Streptococcus spp. Enterococcus spp. Bile Esculin (growth with esculin hydrolysis) + Catalase - + Micrococcus spp. Staphylococcus spp. Streptococcus pneumoniae Streptococcus mitis Streptococcus pyogenes Group A Streptococcus spp. Group B Enterococcus spp. Streptococcus spp. Enterococcus faecalis Enterococcus faecium Enterococcus hirae Enterococcus mundtii Enterococcus spp. Streptococcus spp. Growth w/ Tellurite Gamma (!) Mannitol Fermentation - + Staphylococcus aureus - Hemolysis Enterococcus faecalis Enterococcus faecium (mannitol pos., white colony) Staphylococcus spp. Micrococcus spp. Enterococcus hirae (mannitol neg.) Enterococcus mundtii (mannitol pos., yellow colony) Beta (') Yellow Pigment (Colony) Alpha (#) + Streptococcus pyogenes Group A Streptococcus spp. Group B - Micrococcus spp. Staphylococcus spp. Pyrrolidonearylamidase (PYR Test) Streptococcus pneumoniae Streptococcus mitis Novobiocin Sensitivity Glucose Fermentation + Micrococcus varians Yes + Streptococcus pyogenes Group A Staphylococcus epidermidis - Micrococcus luteus Streptococcus spp. Group B (Streptococcus agalactiae) agalactiae ) No Staphylococcus saprophyticus Optochin Sensitivity Yes Strept Streptoco ococcu ccuss pneumo pneumonia niaee 5 No Strept Streptoco ococcu ccuss mitis mitis Identification flow charts Gram Negative Rods ID Flowchart Gram Negative Rods Oxidase Test + Enterobacteriaceae (See Separate Flowchartt) Aeromonas, Pseudomonas, Vibrio + Acid Vibrio spp., Aeromonas spp., Aeromonas spp. - Glucose Fermentation - Acid Pseudomonas spp. Fluorescent Diffusible Yellow Pigment Na+ Required for Growth (Fluorescein)—use (Fluorescein)—use Psuedo F Agar Aeromonas Aeromonas spp. + + VP Vibrio spp. - + A. hydrophila A. salmonicida Luminescent Other Aeromonas Aeromonas spp. (See Bergey's) (subsp. masoucida) - P. P. aeruginosa P. chlororaphis P. cichorii P. fluorescens P. putida P. syringae Other Pseudomonas Pseudomonas spp. (See Bergey's) (Oxidase Neg.) A. sobria (no gas from glucose) A. veronii + - V. fischeri V. logei V. orientalis V. splendidius Non-Fluorescent Non-Fluorescent Diffusible Blue Pigment Other Vibrio Other Vibrio spp. (See Bergey's) (Pyocyanin)—use Psuedo P Agar Motility + Pigment + (Yellow Colony) A. hydrophila A. veronii + - V. fischeri V. logei V. orientalis V. splen didius A. salmonicida (subsp. masoucida) P. chlororaphis P. cichorii P. fluorescens P. putida P. syringae P. aeruginosa H2S Production Nitrate Reduction + A. hydrophila 30°C Growth A. veronii + 37°C Growth + V. fischeri - P. chlororaphis + V. logei V. orientalis (Colony Pigmented) P. fluorescens - (To differentiate Biovars do a lecithinase test) V. sp lendidius 6 P. putida (Lecithinase Neg.) P. syringae (Oxidase Neg.) P. cichorii (Lecithinase Pos.) Identification flow charts Family Enterobacteriaceae Lactose Positive ID Flowchart Family Enterobacteriaceae Lactose Fermentation + Citrobacter diversus Citrobacter freundii Enterobacter aerogenes Enterobacter cloacae Enterobacter amnigenus Enterobacter intermedius Erwinia carotovora - Erwinia chrysanthemi Escherichia coli Klebsiella oxytoca Klebsiella pneumoniae See Lactose Negative Flowchart for Enterobacteriaceae Enterobacteriaceae (subsp. ozaenae and pneumoniae) Serratia fonticola Serratia rubidaea Indole The Rest + Citrobacter diversus Escherichia coli Erwinia chrysanthemi Klebsiella oxytoca MR-VP -/+ +/+ Klebsiella pneumoniae (subsp. pneumoniae) Enterobacter intermedius +/Citrobacter freundii Serratia fonticola Klebsiella pneumoniae Citrate Enterobacter aerogenes Enterobacter cloacae Enterobacter amnigenus Erwinia carotovora Serratia rubidaea (subsp. ozaenae) Lysine Decarboxylase + + - Citrobacter diversus Escherichia coli subsp. pneumoniae Klebsiella pneumoniae subsp. pneumoniae Enterobacter cloacae Non-motile, No Pigment (VP neg.) Serratia rubidaea Erwinia chrysanthemi Acid from sorbitol Enterobacter amnigenus Motile, Red pigment (VP and H2S pos.) No acid from sorbitol Enterobacter aerogenes H2S Production Klebsiella oxytoca (VP pos and H2S neg.) Motile, No pigment ?? Erwinia carotovora Lysine unknown but Gelatinase positive; none above are positive. + Citrobacter freundii Serratia fonticola Motility pos. Klebsiella pneumoniae subsp. ozaenae Motility neg. 7 Identification flow charts Family Enterobacteriaceae Lactose Negative ID Flowchart Family Enterobacteriaceae Continued Lactose Negative Flow Chart Edwardsiella tarda Erwinia cacticida Morganella morganii Proteus mirabilis Proteus penneri Proteus vulgaris Providencia stuartii Salmonella bongori Salmonella enterica Edwardsiella tarda Morganella morganii Proteus vulgaris Providencia stuartii Serratia marcescens Serratia liquefaciens flexneri) Shigella spp. spp. (boydii, dysenteriae, flexneri) Shigella sonnei Yersinia enterocolitica Yersinia pestis Yersinia pseudotuberculosis Erwinia cacticida Proteus mirabilis Proteus penneri Salmonella bongori Salmonella enterica Serratia marcescens Serratia liquefaciens Shigella sonnei Yersinia pestis Yersinia pseudotuberculosis - + Indole Test Urease + ?? Please See Bergey’s to ID: Shigella spp. Erwinia cacticida Salmonella bongori Salmonella enterica Serratia marcescens Serratia liquefaciens Shigella sonnei Yersinia pestis Proteus mirabilis Ornithine decarb. pos. flexneri) (boydii, dysenteriae, flexneri) Proteus penneri Yersinia enterocolitica Ornithine decarb. neg., motile H2S Yersinia pseudotuberculosis Ornithine decarb. neg., non-motile + - Edwardsiella tarda Proteus vulgaris Morganella morganii Providencia stuartii Motility + - Erwinia cacticida Shigella sonnei Yersinia pestis (lysine decarb. neg., the rest pos.) Urease Salmonella bongori Salmonella choleraesuis Serratia marcescens Serratia liquefaciens Ornithine Decarboxylase + + Proteus vulgaris Providencia stuartii Ornithine Decarboxylase Edwardsiella tarda Morganella morganii H2S + Shigella sonnei + - Salmonella bongori Serratia marcescens KCN growth pos. Red pigment Salmonella enterica KCN growth neg. Serratia liquefaciens No Red pigment (It is this one) 8 Yersinia pestis
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Identification flow charts 1 Bergey’s Manual of Determinative Bacteriology All of the unknowns will fall into the following groups in Bergey's Manual of Determinative Bacteriology (The pink book on the shelf in the laboratory). GROUP 4 Description: Gram Negative, Aerobic/Microaerophilic rods and cocci. A number of important changes occurred at Bergey’s Manual Trust during the preparation of this volume. In 2006, George Garrity retired from the Trust, and the Trust moved its offices from Michigan State University to the University of Georgia. Bergey's Manual of Systematic Bacteriology is the main resource for determining the identity of prokaryotic organisms, emphasizing bacterial species, using every characterizing aspect. The manual was published subsequent to the Bergey's Manual of Determinative Bacteriology, though the latter is still published as a guide for identifying unknown bacteria. Bergey’s manual of bacterial classification 1. Bergey’s manual of bacterial classification Presented by: Kanta Yadav Noopur Joshi Department of: Biotechnology 2. INTRODUCTION TO MICROBIAL TAXONOMY AND CLASSIFICATION 3. TAXONOMY. Is defined as the science of biological classification.

Transcript

Bergey’s Manual of Determinative Bacteriology
All of the unknowns will fall into the following groups in Bergey's Manual of Determinative
Bacteriology (The pink book on the shelf in the laboratory).

GROUP 4
Description: Gram Negative, Aerobic/Microaerophilic rods and cocci
Key differences are: pigments/fluorescent, motility, growth requirements, denitrification,
morphology, and oxidase, read Genera descriptions
Examples: Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter

GROUP 5
Description: Facultatively Anaerobic Gram negative rods
Key differences are: growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions
Examples: Family Enterobacteriaceae and Vibrionaceae

GROUP 17
Description: Gram-Positive Cocci
Key differences are: oxygen requirements, morph., growth requirements (45°C and supplements),
read Genera descriptions
Examples: Micrococcus, Staphylococcus, Streptococcus, Enterococcus, Lactococcus, Aerococcus

GROUP 18
Description: Endospore-Forming Gram positive rods and cocci
Key differences are: oxygen requirements, motility, morph, catalase
Examples: Bacillus, Clostridium

GROUP 19
Description: Regular, Nonsporlating Gram positive rods
Key differences are: morph., oxygen require, catalase
Examples: Lactobacillus, Listeria

GROUP 20
Description: Irregular, Nonsporlating Gram-positive rods
Key differences are: catalase, motility, morph., read Genera descriptions
Examples: Actinomyces, Corynebacterium, Arthrobacter, Propionibacterium

GROUP 21
Description: Weakly Gram-Positive Nonsporlating Acid Fast Slender Rods
Key differences are: acid fast, growth
Examples: Mycobacterium

1
Identification flow charts

Differentiation via Gram stains and cell morphology.

Gram Stain & Morphological
Flowchart
Some Examples

Gram Positive
Cocci Bacilli

Filamentous
Round in clusters Oval shape in Club-shaped and/or in Spore-bearing,
& tetrads: Chains: palisades: large, uniform:
Staphylococcus Streptococcus Corynebacterium Bacillus
Micrococcus Peptostreptococcus Listeria Clostridium Extensive Branching rudi-
Peptococcus Enterococcus Erysipelothrix branching: mentry or absent:
Mycobacterium (Acid Fast) Actinomyces Erysipelothrix
Propionobacterium Arachnia Lactobacillus
Nocardia (partially Eubacterium
acid-fast)
Streptomyces

Gram Negative

Cocci Bacilli

Neisseria Medium-sized Tiny-cocco- Pleomorphic Exaggerated Curved, Uniformly
Veillonella cocco-bacillary: bacillary: coccobacillary pointed ends: comma-shaped: Bacillary:
Acinetobacter Brucella & filamentous: Fusobacterium Vibrio Enterobacteriaceae
Moraxella Bordetella Haemophilus nucleatum Campylobacter Pseudomonas
Bacteroides Bacteroides Spirillium Aeromonas
Pasteurella Alcaligenes
Francisella Chromobacterium
Actinobacillus Coiled &
Eikenella sphero-plastic:
Cardiobacterium Streptobacillus
Flavobacterium Fusobacterium

2
Identification flow charts
Gram Positive Rods ID Flowchart

Gram Positive Rods

Bacillus spp.
Clostrididium spp.
Corynebacterium spp.
Lactobacillus spp.
Mycobacterium spp.

Mycobacterium smegmatis
Spore Forming

+ - +

Bacillus spp. Corynebacterium spp.
Clostridium spp Lactobacillus spp. Acid Fast
Mycobacterium spp.

-
Strict Anaerobes Corynebacterium spp
Lactobacillus spp.
+ -

Clostridium spp. Bacillus spp.
You sure you have this? See separate flowchart.
Go to Bergey's. Catalase

+ -
Corynebacterium spp. Lactobacillus spp.

Manual

Starch Hydrolysis

Glucose Ferm. Activity

+ -

Acid & Gas Acid
Corynebacterium kutsceri Corynebacterium xerosis
Lactobacillus fermenti Lactobacillus casei
Lactobacillus delbrueckii

Mannitol

+ -
Lactobacillus casei Lactobacillus delbrueckii

3
Identification flow charts
Bacillus spp. ID Flowchart

Bacillus spp.
Starch Hydrolysis B. pumilus
(amylase) (VP is pos., rest are neg.)
B. subtilis
B. marinus
B. cereus - B. sphaericus
B. azotoformans
B. megaterium B. larvae
B. stearothermophilus B. schlegelii B. pasteurii
B. polymyxa
+ (grows at 55°C, rest no) (round spore, rest
B. popilliae are oval)
B. mycoides VP B. pasteurii B. popilliae
B. macerans (add creatine as catalyst B. lentimorbus
B. thuringiensis w/ reagents) -
B. azotoformans
B. macquariensis
B. badius
B. licheniformis
B. insolitus
B. lentus + - B. larvae
B. alvei
B. subtilis B. megaterium B. laterosporus Catalase Citrate
B. anthracis
B. alcalophilus B. cereus B. stearothermophilus
B. coagulans B. polymyxa B. macerans
B. mycoides B. pantothenticus + +
B. brevis
B. macquariensis B. badius -
B. circulans B. thuringiensis
B. licheniformis B. lentus (spore is not
B. firmus round, rest are) B. azotoformans B. larvae
B. pantothenticus B. alvei B. alcalophilus (gelatinase pos.)
B. badius B. insolitus
B. anthracis B. popilliae
B. brevis B. laterosporus
B. coagulans (gelatinase neg.)
B. circulans B. pasteurii
B. marinus
B. sphaericus
Cell Diamenter ! 1'm Swollen Cell Swollen Cell
(the width) (containing spore) (containing spore)

+ - + -
B. anthracis B. subtilis B. stearothermophilus B. megaterium + -
(non-motile) B. polymyxa (growth at 55°C-rest neg.) (citrate pos.)
B. thuringiensis B. lichenifomis B. macerans B. badius B. sphaericus B. insolitus
(makes insecticidal B. alvei B. pantothenticus (citrate neg.) B. pasteurii (acid from glucose)
protein)
B. coagulans B. macquariensis B. laterosporus B. marinus
B. mycoides B. lentus (oval spore, (acid from glucose)
(colony has a (catalase neg.-rest pos.) rest are round)
rhizoidal appearance) B. alcalophilus
B. cereus B. brevis
(Motile - It is this B. circulans
one ) Nitrate Reduction

Citrate + -
6.5% NaCl Growth B. pasteurii B. sphaericus
+ -
B. coagulans B. polymyxa + -
B. licheniformis (manitol pos.)
B. subtilis B. alvei B. macerans B. pantothenticus
(manitol neg.) B. macquariensis (acid via arabinose neg.)
B. alcalophilus B. circulans
B. brevis (acid via arabinose pos.)
B. circulans

6.5% NaCl Growth

B. macerans
+ - Acid from Arabinose
+ (gas from glucose pos.)
B. alcalophilus
B. subtilis B. coagulans - (gas from glucose neg.,
(no growth at 55°C) no growth < pH 7.0)
B. licheniformis B. macquariensis B. circulans
(growth at 55°C) (methyl red pos.) (gas from glucose neg.,
B. brevis growth < pH 7.0)
(methyl red neg.)

4
Identification flow charts
Gram Positive Cocci ID Flowchart

Gram Positive Cocci
Streptococcus spp.
Micrococcus spp. - (see Bergey’s)
Staphylococcus spp.
Streptococcus spp. Bile Esculin
Enterococcus spp. (growth with esculin hydrolysis) Enterococcus faecalis
Enterococcus faecium
Enterococcus hirae
+ Enterococcus mundtii
Catalase -
Streptococcus pneumoniae
Streptococcus mitis
Streptococcus pyogenes Group A
+ Streptococcus spp. Group B
Enterococcus spp. Enterococcus spp.
Micrococcus spp. Streptococcus spp. Streptococcus spp.
Staphylococcus spp.
Growth w/ Tellurite
Gamma (!)

Mannitol Fermentation
+ -
- Hemolysis
+ Enterococcus faecalis Enterococcus faecium
Staphylococcus aureus Staphylococcus spp. (mannitol pos., white colony)
Micrococcus spp. Enterococcus hirae
(mannitol neg.)
Enterococcus mundtii
(mannitol pos., yellow colony)
Beta (')
Yellow Pigment (Colony)
Alpha (#)
Streptococcus pyogenes Group A
+ - Streptococcus spp. Group B
Micrococcus spp. Staphylococcus spp.

Pyrrolidonearylamidase
(PYR Test)

Glucose Fermentation Novobiocin Sensitivity Streptococcus pneumoniae
Streptococcus mitis

+
Micrococcus varians Yes +
Streptococcus pyogenes Group A
Staphylococcus epidermidis -
- - No Streptococcus spp. Group B
(Streptococcus agalactiae)
Micrococcus luteus Staphylococcus saprophyticus

How to cite bergey

Optochin Sensitivity

Yes No

Streptococcus pneumoniae Streptococcus mitis

5
Identification flow charts
Gram Negative Rods ID Flowchart

Gram Negative Rods
Oxidase Test -
+
Enterobacteriaceae
Aeromonas, Pseudomonas, Vibrio (See Separate Flowchartt)

+ Acid - Acid
Vibrio spp., Aeromonas spp. Glucose Fermentation Pseudomonas spp.

Fluorescent Diffusible Yellow Pigment
Na+ Required for Growth (Fluorescein)—use Psuedo F Agar
-
Aeromonas spp.

+ -
+
VP P. aeruginosa Other Pseudomonas spp.
Vibrio spp. P. chlororaphis (See Bergey's)
- P. cichorii
+
P. fluorescens
A. hydrophila Other Aeromonas spp. P. putida
A. salmonicida (See Bergey's) P. syringae
Luminescent (subsp. masoucida) (Oxidase Neg.)
A. sobria
(no gas from glucose)
A. veronii
+ -
Non-Fluorescent Diffusible Blue Pigment
V. fischeri Other Vibrio spp. (Pyocyanin)—use Psuedo P Agar
V. logei (See Bergey's)
V. orientalis
V. splendidius Motility

- +
P. chlororaphis P. aeruginosa
- P. cichorii
Pigment + P. fluorescens
(Yellow Colony) A. hydrophila P. putida
A. salmonicida
A. veronii (subsp. masoucida) P. syringae

+ -
V. fischeri V. orientalis
V. logei V. splendidius
H2S Production
Nitrate Reduction

+ -
A. hydrophila A. veronii
+ -
30°C Growth 37°C Growth
P. putida
P. chlororaphis
+ + (Colony Pigmented)
(Lecithinase Neg.)
P. syringae
V. fischeri V. orientalis P. fluorescens (Oxidase Neg.)
- - (To differentiate Biovars
do a lecithinase test)
P. cichorii
V. logei V. splendidius (Lecithinase Pos.)

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6
Identification flow charts
Family Enterobacteriaceae Lactose Positive ID Flowchart

Family Enterobacteriaceae
Lactose Fermentation

+ -

Bergey's Manual online, free

Citrobacter diversus Erwinia chrysanthemi See Lactose Negative Flowchart
Citrobacter freundii Escherichia coli for Enterobacteriaceae
Enterobacter aerogenes Klebsiella oxytoca
Enterobacter cloacae Klebsiella pneumoniae
Enterobacter amnigenus (subsp. ozaenae and pneumoniae)
Enterobacter intermedius Serratia fonticola
Erwinia carotovora Serratia rubidaea

Indole

-
The Rest
+
Citrobacter diversus
Escherichia coli
Erwinia chrysanthemi
MR-VP
Klebsiella oxytoca +/+ -/+ Klebsiella pneumoniae
(subsp. pneumoniae)
Enterobacter intermedius Enterobacter aerogenes
Enterobacter cloacae
Enterobacter amnigenus
+/- Erwinia carotovora
Citrobacter freundii Serratia rubidaea
Citrate Serratia fonticola
Klebsiella pneumoniae
(subsp. ozaenae)

Lysine Decarboxylase

+ -
+ -
Klebsiella pneumoniae subsp. pneumoniae Enterobacter cloacae
Citrobacter diversus Escherichia coli Non-motile, No Pigment Acid from sorbitol
(VP neg.) Serratia rubidaea Enterobacter amnigenus
Erwinia chrysanthemi Motile, Red pigment No acid from sorbitol
(VP and H2S pos.) Enterobacter aerogenes
Klebsiella oxytoca H2S Production Motile, No pigment
(VP pos and H2S neg.)

??

Erwinia carotovora
Lysine unknown but Gelatinase
positive; none above are positive.
+ -
Citrobacter freundii Serratia fonticola
Motility pos.
Klebsiella pneumoniae subsp. ozaenae
Motility neg.

7
Identification flow charts
Family Enterobacteriaceae Lactose Negative ID Flowchart

Bergey's Manual Of Determinative Microbiology

Family Enterobacteriaceae Continued
Lactose Negative Flow Chart
Erwinia cacticida
Proteus mirabilis
Edwardsiella tarda Proteus penneri
Erwinia cacticida Serratia marcescens Salmonella bongori
Morganella morganii Serratia liquefaciens Salmonella enterica
Proteus mirabilis Shigella spp. (boydii, dysenteriae, flexneri) Serratia marcescens
Proteus penneri Shigella sonnei Serratia liquefaciens
Proteus vulgaris Yersinia enterocolitica Shigella sonnei
Providencia stuartii Yersinia pestis - Yersinia pestis
Salmonella bongori Yersinia pseudotuberculosis Yersinia pseudotuberculosis
Salmonella enterica

+ Indole Test
Edwardsiella tarda
Morganella morganii
Urease
Proteus vulgaris
Providencia stuartii ?? + -
Please See Bergey’s to ID: Erwinia cacticida
Shigella spp. Proteus mirabilis
(boydii, dysenteriae, flexneri) Ornithine decarb. pos. Salmonella bongori
Yersinia enterocolitica Proteus penneri Salmonella enterica
Ornithine decarb. neg., motile Serratia marcescens
H2S Yersinia pseudotuberculosis Serratia liquefaciens
Ornithine decarb. neg., non-motile Shigella sonnei
Yersinia pestis

+ -
Edwardsiella tarda Morganella morganii
Motility
Proteus vulgaris Providencia stuartii
+ -
Erwinia cacticida Shigella sonnei
(lysine decarb. neg., the rest pos.) Yersinia pestis
Urease Ornithine Decarboxylase Salmonella bongori
Salmonella choleraesuis
Serratia marcescens
Serratia liquefaciens
+ +
Proteus vulgaris Providencia stuartii
Ornithine Decarboxylase
- -
Edwardsiella tarda Morganella morganii
H2S

+
Shigella sonnei -
+ -
Yersinia pestis
Salmonella bongori Serratia marcescens
KCN growth pos. Red pigment
Salmonella enterica Serratia liquefaciens
KCN growth neg. No Red pigment
(It is this one)

Bergey's Manual Chart

8

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